abstract：:The metabolic engineering of natural products has begun to prosper in the past few years due to genomic research and the discovery of biosynthetic genes. While the biosynthetic pathways and genes for some isoprenoids have been known for many years, new pathways have been found and known pathways have been further inve...

journal_title：Metabolic engineering

authors： Barkovich R,Liao JC

更新日期：2001-01-01 00:00:00

abstract：:Metabolic flux analysis is a useful tool to analyze cell metabolism. In this study, we report the use of a metabolic model with 34 fluxes to study the 293 cell, in order to improve its growth capacity in a DMEM/F12 medium. A batch, fed-batch with glutamine feeding, fed-batch with essential amino acids, and finally a f...

journal_title：Metabolic engineering

authors： Nadeau I,Sabatié J,Koehl M,Perrier M,Kamen A

更新日期：2000-10-01 00:00:00

abstract：:Global gene regulation throughout the Escherichia coli stress response to overexpression of each of five recombinant proteins was evaluated. Reverse-transcriptase polymerase chain reaction-amplified mRNA from induced and control cells were hybridized with a DNA array of Kohara clones representing 16% (700 genes) of th...

journal_title：Metabolic engineering

authors： Gill RT,Valdes JJ,Bentley WE

更新日期：2000-07-01 00:00:00

abstract：:Previous work in our laboratories investigated the use of methyl alpha-glucoside (alpha-MG), a glucose analog that shares a phosphotransferase system with glucose, to modulate glucose uptake and therefore reduce acetate accumulation. The results of that study showed a significant improvement in batch culture performan...

journal_title：Metabolic engineering

authors： Berríos-Rivera SJ,Yang YT,Bennett GN,San KY

更新日期：2000-04-01 00:00:00

abstract：:One of the important goals of biology is to understand the relationship between DNA sequence information and nonlinear cellular responses. This relationship is central to the ability to effectively engineer cellular phenotypes, pathways, and characteristics. Expression arrays for monitoring total gene expression based...

journal_title：Metabolic engineering

authors： Hatzimanikatis V,Lee KH

更新日期：1999-10-01 00:00:00

abstract：:The study was aimed at evaluating the extent of flux control exercised by the amino acid excretion step on the glutamate production flux in C. glutamicum 2262 strain that is induced for glutamate excretion by an upward temperature shift. Cells initially induced to excrete glutamate were cultivated at different control...

journal_title：Metabolic engineering

authors： Lapujade P,Goergen JL,Engasser JM

更新日期：1999-07-01 00:00:00

abstract：BACKGROUND:6-Methylsalicylic acid synthase (MSAS), a fungal polyketide synthase from Penicillium patulum, is perhaps the simplest polyketide synthase that embodies several hallmarks of this family of multifunctional enzymes--a large multidomain protein, a high degree of specificity toward acetyl-CoA and malonyl-CoA sub...

journal_title：Metabolic engineering

authors： Richardson MT,Pohl NL,Kealey JT,Khosla C

更新日期：1999-04-01 00:00:00

abstract：:Metabolic engineering is the directed improvement of cellular properties through the modification of specific biochemical reactions or the introduction of new ones, with the use of recombinant DNA technology. As such, metabolic engineering emphasizes metabolic pathway integration and relies on metabolic fluxes as dete...

journal_title：Metabolic engineering

authors： Stephanopoulos G

更新日期：1999-01-01 00:00:00